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Pyrosequencing Inc quantitative bisulfite pyrosequencing method
Quantitative Bisulfite Pyrosequencing Method, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+bisulfite+pyrosequencing/bisulfite+pyrosequencing/pm40543411-63-12-13
Average 90 stars, based on 1 article reviews
quantitative bisulfite pyrosequencing method - by Bioz Stars, 2026-10
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Article Title: Systematic review and feasibility study on pre-analytical factors and genomic analyses on archival formalin-fixed paraffin-embedded breast cancer tissue
Article Snippet: Therefore, nucleic acid analysis technique platforms like other PCR-based methods (e.g., qPCR, RT-qPCR, hydrolysis of pre-existing sequences TaqMan ® ), DNA methylation analysis methods (e.g., quantitative bisulfite pyrosequencing), chromatin immunoprecipitation sequencing (i.e., ChIP-Seq) were not included in our study.

Expressing:

Article Title: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
Article Snippet: .. Results showed that lower expression of miR-342-3p was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.074; Fig. a) or by quantitative bisulfite pyrosequencing ( P = 0.024, Fig. ). .. Similarly, the lower expression of EVL was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.046; Fig. c) or by quantitative bisulfite pyrosequencing ( P = 0.001; Fig. d).

Article Title: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
Article Snippet: Results showed that lower expression of miR-342-3p was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.074; Fig. a) or by quantitative bisulfite pyrosequencing ( P = 0.024, Fig. ). .. Similarly, the lower expression of EVL was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.046; Fig. c) or by quantitative bisulfite pyrosequencing ( P = 0.001; Fig. d). ..

Methylation:

Article Title: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
Article Snippet: .. Results showed that lower expression of miR-342-3p was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.074; Fig. a) or by quantitative bisulfite pyrosequencing ( P = 0.024, Fig. ). .. Similarly, the lower expression of EVL was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.046; Fig. c) or by quantitative bisulfite pyrosequencing ( P = 0.001; Fig. d).

Article Title: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
Article Snippet: .. This was associated with promoter DNA demethylation of E-CAD, which was evidenced by the emergence of U-MSP signal (Fig. c) and reduction of mean methylation level from 96.57% to 84.57% using quantitative bisulfite pyrosequencing (Additional file : Figure S2), and re-expression of E-CAD by qRT-PCR (Fig. d). ..

Article Title: MicroRNA Methylome Signature and Their Functional Roles in Colorectal Cancer Diagnosis, Prognosis, and Chemoresistance
Article Snippet: miR-132 [ ] Known targets: miR-132: ZEB2, ERK1 , Methylation-specific PCR (MSP) and bisulfite sequencing , CRC cell lines and 36 CRCs with adjacent normal tissues , miR-132 is epigenetically silenced in CRC cell lines and implies a poor prognosis in CRC. .. miR-1, miR-9, miR-124, miR-137 [ ] Known targets: miR-1: SMAD3 miR-9: TM4SF1, FOXP2, ANO1 miR-124: STAT3, IASPP PRRX1, KITENIN, PRPS1, RPIA. PTB1/PKM1/PKM2, DNMT3B, DNMT1, ROCK1, PRRX1, PLCB1. miR-137: TCF4, FMNL2, Aurora-A , Quantitative bisulfite pyrosequencing , 387 colorectal epithelial specimens (362 non-neoplastic and 25 neoplastic tissues) , Among patients with ulcerative colitis without neoplasia, the rectal tissues had significantly higher levels of microRNA methylation Methylation level was associated with age and duration of ulcerative colitis. .. miR-125 [ ] Known targets: BCL2, BCL2L12, MCL1, SMURF1, VEGFA, TAZ, CXCL12/CXCR4 , Bisulfite sequencing PCR , CRC tissues and adjacent normal tissues from 68 CRC patients , Patients with hypermethylation of miR-125a and miR-125b had a shorter life expectancy than those with normal levels.

Article Title: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
Article Snippet: Results showed that lower expression of miR-342-3p was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.074; Fig. a) or by quantitative bisulfite pyrosequencing ( P = 0.024, Fig. ). .. Similarly, the lower expression of EVL was associated with EVL/MIR342 methylation by MSP (MM + MU vs. UU, P = 0.046; Fig. c) or by quantitative bisulfite pyrosequencing ( P = 0.001; Fig. d). ..

Article Title: Methylation of the serotonin reuptake transporter gene and non-motor symptoms in dystonia patients.
Article Snippet: .. Methylation of 20 CpG sites situated 69 to 213 base pairs upstream of the start codon of SLC6A4 was investigated by quantitative bisulfite pyrosequencing (Additional file 1: Fig. 1). ..

Article Title: Association of DNA Methylation with Infant Birth Weight in Women with Gestational Diabetes
Article Snippet: Nomura, 2014 [ ] , 7/21 , Placenta and CB , Global , Luminometric Methylation Assay , GDM was associated with placental hypomethylation. Suggestive negative associations were found between placental global methylation and infant body length and head circumference.. .. Lesseur, 2014 [ ] , 47/432 , Placenta , LEP promoter region , Quantitative bisulfite pyrosequencing , Placentas from infants exposed to GDM had higher DNAm compared to those from the non-GDM group. LEP methylation was not associated with infant birth weight.. .. Allard, 2015 [ ] , 173 , CB , LEP genomic region , Human Methylation 450 BeadChips at LEP locus , Increased maternal glycemia associated with lower methylation levels at LEP locus. Lower DNAm at LEP locus associated with higher leptin levels in CB and higher birth weight..

Quantitative RT-PCR:

Article Title: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
Article Snippet: .. This was associated with promoter DNA demethylation of E-CAD, which was evidenced by the emergence of U-MSP signal (Fig. c) and reduction of mean methylation level from 96.57% to 84.57% using quantitative bisulfite pyrosequencing (Additional file : Figure S2), and re-expression of E-CAD by qRT-PCR (Fig. d). ..



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Quantitative methylation analysis of HOXA11 promoter in cervical scrapes (n=387) representing normal, various cervical dysplasia and biopsy-confirmed invasive cervical cancer. Average methylation percentages on the HOXA11 promoter are represented by box and whisker plots.

Journal: Development & Reproduction

Article Title: Overexpression of Hypermethylated Homeobox A11 (HOXA11) Inhibits Tumor Cell Growth and Induces Apoptosis in Cervical Cancer

doi: 10.12717/DR.2024.28.2.37

Figure Lengend Snippet: Quantitative methylation analysis of HOXA11 promoter in cervical scrapes (n=387) representing normal, various cervical dysplasia and biopsy-confirmed invasive cervical cancer. Average methylation percentages on the HOXA11 promoter are represented by box and whisker plots.

Article Snippet: To assess methylation status of HOXA11 gene, quantitative bisulfite-pyrosequencing ( ) was performed.

Techniques: Methylation, Whisker Assay

Cervical cancer cells were seeded on 24-well culture plates and attached for 24 hours. HeLa and HT3 cancer cells were transiently transfected with expression vectors encoding HOXA11 and the number of survival cells was counted at 1 day intervals for 3 days. Results are means±SD from three samples each. The cells were observed at 40× magnification.

Journal: Development & Reproduction

Article Title: Overexpression of Hypermethylated Homeobox A11 (HOXA11) Inhibits Tumor Cell Growth and Induces Apoptosis in Cervical Cancer

doi: 10.12717/DR.2024.28.2.37

Figure Lengend Snippet: Cervical cancer cells were seeded on 24-well culture plates and attached for 24 hours. HeLa and HT3 cancer cells were transiently transfected with expression vectors encoding HOXA11 and the number of survival cells was counted at 1 day intervals for 3 days. Results are means±SD from three samples each. The cells were observed at 40× magnification.

Article Snippet: To assess methylation status of HOXA11 gene, quantitative bisulfite-pyrosequencing ( ) was performed.

Techniques: Transfection, Expressing

HeLa cells were transiently transfected with control pcDNA3.1 and pcDNA3.1-HOXA11. (A) Mitochondrial and cytosolic fractions were collected at 48 hrs after transfection and quantified cytochrome C concentration by western hybridization. (B) HeLa cells were transiently transfected with pcDNA3.1-HOXA11, then harvested at 24 hrs and 48 hrs after transfection and analyzed by western blot. The expression levels of HOXA11, Bax, cleaved PARP, c-Myc, VEGF, and GAPDH were measured.

Journal: Development & Reproduction

Article Title: Overexpression of Hypermethylated Homeobox A11 (HOXA11) Inhibits Tumor Cell Growth and Induces Apoptosis in Cervical Cancer

doi: 10.12717/DR.2024.28.2.37

Figure Lengend Snippet: HeLa cells were transiently transfected with control pcDNA3.1 and pcDNA3.1-HOXA11. (A) Mitochondrial and cytosolic fractions were collected at 48 hrs after transfection and quantified cytochrome C concentration by western hybridization. (B) HeLa cells were transiently transfected with pcDNA3.1-HOXA11, then harvested at 24 hrs and 48 hrs after transfection and analyzed by western blot. The expression levels of HOXA11, Bax, cleaved PARP, c-Myc, VEGF, and GAPDH were measured.

Article Snippet: To assess methylation status of HOXA11 gene, quantitative bisulfite-pyrosequencing ( ) was performed.

Techniques: Transfection, Control, Concentration Assay, Western Blot, Hybridization, Expressing

Summary of DNA methylation studies in AD

Journal: Journal of Alzheimer's Disease Reports

Article Title: Utility of DNA Methylation as a Biomarker in Aging and Alzheimer’s Disease

doi: 10.3233/ADR-220109

Figure Lengend Snippet: Summary of DNA methylation studies in AD

Article Snippet: AD , Peripheral blood , 83 (43 case, 38 control) , 55-87 , Quantitative bisulfite-PCR pyrosequencing , Targeted repetitive elements , LINE-1 methylation increased in AD compared to controls , [ ] .

Techniques: DNA Methylation Assay, Southern Blot, Control, Immunohistochemistry, Methylation, Mass Spectrometry, Methylation Sequencing, Biomarker Discovery, Fluorescence, High Content Screening, Enzyme-linked Immunosorbent Assay, Cloning, Sequencing, Gene Expression, Luciferase